Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan, Shandong, China (People's Republic)
Introduction: Immune disorders caused by sepsis have recently drawn much attention. We sought to dynamically monitor the expression of exosome miRNAs in peripheral blood during sepsis to explore these miRNAs as potential biomarkers for monitoring immune function in sepsis patients.
Methods: This study included patients with sepsis. Blood samples were obtained from 10 patients on the first through 10th days, the 12th day, and the 14th day since sepsis onset, resulting in 120 collected samples. Serum exosomes were extracted from peripheral venous blood, and levels of MIR497HG, miR-195, miR-497, and PD-L1 in serum exosomes were detected by qPCR, and clinical information was recorded.
Results: Our study revealed that the levels of MIR497HG, miR-195, miR-497, and PD-L1 in serum exosomes showed periodic changes; the time from peak to trough was approximately 4-5 days. The levels of exosome MIR497HG and miR-195 had a positive linear relationship with SOFA score (r values were -0.181 and -0.189; P values were 0.048 and 0.039, respectively). The recorded quantities of exosome MIR497HG, miR-195, and PD-L1 showed a substantial correlation with ARDS. ROC curve analysis revealed that exosome MIR497HG, miR-195, and miR-497 could predict the 28-day mortality of sepsis patients with an AUC of 0.66, 0.68, and 0.72, respectively.
Conclusions: Levels of exosomes MIR497HG, miR-195, miR-497, and PD-L1 showed periodic changes with the immune status of sepsis, which provides a new exploration direction for immune function biomarkers and immunotherapy timing in sepsis patients.
